|
KMID : 0381219840160030258
|
|
Journal of RIMSK
1984 Volume.16 No. 3 p.258 ~ p.265
|
|
|
The Study for Cytochemical Reaction in Acute Leukemia
|
|
|
|
|
Abstract
|
|
|
According to recent advance in chemotherapy of leukemia, accurate identification of cell. types has become a necessity practically.
Cytochemistry has widely accepted as a supplement to Romanowsky staining for the classification of acute leukemia after a proposal of FAB-classification.
In order to establish guidelines for categorization of subclasses, we attempted six special cytochemical studies.
Material from 21 patients (13 acute myeloid leukemia, 8 acute lymphoblastic leukemia) were studied by 6 special cytochemical reaction for identification of acute leukemia according to FAB classification at the Department of Clinical Pathology, Chung Ang University Hospital, from January 1983 to May 1984.
The results are summerized as follows:
1) Eleven among 12 AML cases were shown PAS positivity and 5 among , ALL cases.
The block or rings of activity is not clearly correlated with ALL.
2) If the positivity in SBB or myeloperoxidase stain is over 25%, ALL can be excluded.
3) Although activity of SBB in AML were more sensitive and stronger than iryeloperoxidase, it is desirable to perform both of two methods complementary.
4) Not only non-specific esterase staining is essential for acute monocytic leukemia diagnosis, but also the combination of non-specific and specific esterase stain represent diagnostic value, in FAB-M4.
5) Although leukocyte acid phosphatase stain is not necessary for all acute leukemia, tartrate-inhibition reaction is required to be performed to determine T-ALL.
6) The cytochemical reaction after chemotherapy is little value for differential diagnosis of leukemic subclassfication.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|